Validation of a method for predicting the precision, limit of detection and range of quantitation in competitive ELISA.

The mathematical model for predicting the precision, limit of detection (LOD) and range of quantitation (ROQ) in a competitive enzyme-linked immunosorbent assay (ELISA) proposed by Hayashi et al. (Anal. Chem., 2004, 76, 1295) was validated. The model describes the relative standard deviation (RSD) of concentration estimates by the RSDs of pipetting volumes of analyte, enzyme-conjugated antigen, antibody and substrate solutions, and the standard deviation (SD) of inherent absorbances between the wells in an ELISA plate. For 6 kinds of direct competitive ELISA kits, the LOD and ROQ predicted by the model agreed well with those obtained by experiments with real samples. It was also confirmed that the model is applicable to the prediction of uncertainty that depends on the pipetting error of the viscous antiserum solution. The model was demonstrated to be useful for estimating the LOD and ROQ of competitive ELISA.     

17N+197Au反应中碎片与中子的符合测量

在3?3?.4MeV/u ¹⁷N束轰击¹⁹⁷Au靶产生的反应中,利用放置于不同角度组合的17个中子探测器(4°—83°)和14个半导体望远镜(2.3°—9.0°)对反应产物碎片与中子进行了符合测量.经对所得实验角分布积分得到Z=3—6元素的同位素产额分布.在参加者-旁观者模型框架下,采用¹⁷N原子核内部的不同密度分布计算了同位素产额分布并与实验数据做比较.     

从旋光度测定谈启发研究式化学教学

以比旋光度的测定为例,讨论了浓度、温度、溶剂、波长等因素对比旋光度的影响,介绍了启发研究式开展有机化学教学与实验的新模式     

Influence of silicon on the microstructure and mechanical properties of Zr-Si-N composite films

A series of Zr-Si-N composite films with different Si contents were synthesized in an Ar and N₂ mixture atmosphere by the bi-target reactive magnetron sputtering method. These films’ composition, microstructure and mechanical properties were characterized by energy dispersive spectroscopy, X-ray diffraction, scanning electron microscopy, atomic force microscopy and nanoindentation. Experimental results revealed that after the addition of silicon, Si₃N₄ interfacial phase formed on the surface of ZrN grains and prevented them from growing up. Zr-Si-N composite films were strengthened at low Si content with the hardness and elastic modulus reaching their maximum values of 29.8 and 352 GPa at 6.2 at% Si, respectively. With a further increase of Si content, the crystalline Zr-Si-N films gradually transformed into amorphous, accompanied with a remarkable fall of films’ mechanical properties. This limited enhancement of mechanical properties in the Zr-Si-N films may be due to the low wettability of Si₃N₄ on the surface of ZrN grains.     

On the Bounded Complex of an Affine Oriented Matroid

We prove that the bounded complex of an affine oriented matroid is pure and collapsible. We also generalize Zaslavsky's central decomposition theorem for hyperplane arrangements to affine oriented matroids.     

Some explicit examples of Hamiltonian minimal Lagrangian submanifolds in complex space forms

In this paper, we find some new explicit examples of Hamiltonian minimal Lagrangian submanifolds among the Lagrangian isometric immersions of a real space form in a complex space form.     

Integrability of det▽u and Evolutionary Wente''s Problem Associated to Heat Operator

In this note, the authors resolve an evolutionary Wente's problem associated to heat equation, where the special integrability of det▽u for u ∈ H1(R2,R2) is used.     

Electronic structure of CeAl2 thin films studied by X-ray absorption spectroscopy

We report X-ray absorption near edge structures (XANES) study of CeAl₂ thin films of various thicknesses, 40-120 nm, at Al K- and Ce L₃-edges. The threshold of the absorption features at the Al K-edge shifts to the higher photon energy side as film thickness decreases, implying a decreased in Al p-orbital charges. On the other hand, from Ce L₃-edge spectra, we observed a decrease in the 5d4f occupancy as the surface-to-bulk ratio increases. The valence of Ce in these thin films, as revealed by the Ce L₃-edge spectral results, is mainly trivalent. From a more detailed analysis we found a small amount of Ce⁴⁺ contribution, which increases with decreasing film thickness. Our results indicate that the surface-to-bulk ratio is the key factor which affects the electronic structure of CeAl₂ thin films. The above observations also suggest that charge transfer from Al to Ce is associated with the decrease of the film thickness.     

SHS等离子喷涂制备FeAl2O4-Al2O3-Fe纳米复合涂层的研究

利用SHS等离子喷涂技术,将经过机械团聚法制备的Fe2O3-Al复合粉体送入等离子焰流,沉积出厚度约为400 μm的复合涂层.利用XRD,SEM 和TEM等检测手段对涂层的成分和组织进行了分析,测定了涂层的显微硬度、断裂韧性以及耐磨性.结果表明涂层为具有纳米结构的FeAl2O4-Al2O3-Fe纳米复合组织;涂层的显微硬度为HV100g870;断裂韧性是普通Al2O3涂层的2倍;无润滑磨损的耐磨性是普通Al2O3涂层的2.5倍.     

复杂像散椭圆光束的轨道角动量的实验研究

提出一种直接测量光束的轨道角动量的方法，其原理是把具有轨道角动量的光束照射到一个金属靶上，使其在光束的角动量作用下发生转动. 通过测量靶转动的角度来计算光束的角动量值. 对几种不同参数的光束的轨道角动量进行了测量，获得了轨道角动量与光束参数之间的关系，实验结果与理论分析较好地符合. 关键词： 复杂像散椭圆光束 轨道角动量 测量